Section on Neural Gene Expression
Oxytocin receptor antibodies
Various western studies performed in SNGE
(Heon-Jin Lee, Geetha Kannan, Matthew Morris, & Scott Young, unpublished)
| Source | MBL | Everest | Novus | Apha Diagnostics | Santa Cruz Biotech |
Santa Cruz Biotech |
SDI | ||
| Cat. # | LS-A244 | EB0783 | NLS244 | OTR11-A | C-20 | H-60 | Custom-made | ||
| Antigen origin | Human | Human | Human | Rat | Human | Human | Mouse | ||
| Epitope location | N-terminus | N-terminus | N-terminus | C-terminal cytoplasmic domain |
C-terminus | C-terminus | C-terminus | ||
| Antigen Seq. | n.e. | 64-74 | n.e. | 20 aa | n.e. | 330-389 | 332-388 | ||
| Host | Rabbit | Goat | Rabbit | Rabbit | Goat | Rabbit | Rabbit | ||
| Western blot | BOILED METHODS | Liver | N/S | N/S | N/B | N/B | N/S | N/S | N/S |
| Forebrain | N/S | N/B | N/S | N/B | N/S | N/D | N/B | ||
| Olfactory Bulb | N/S | N/B | N/S | N/B | N/S | N/D | N/B | ||
| NON BOILED METHODS | Liver | n.e. | n.e. | n.e. | n.e. | n.e. | n.e. | N/B | |
| Forebrain | n.e. | n.e. | n.e. | n.e. | n.e. | n.e. | N/S | ||
| Olfactory Bulb | n.e. | n.e. | n.e. | n.e. | n.e. | n.e. | N/S | ||
| Transfected CHO cells (Perkin-Elmer) |
n.e. | n.e. | n.e. | n.e. | n.e. | n.e. | Specific | ||
| Epigen expression | n.e. | n.e. | n.e. | n.e. | n.e. | n.e. | Specific | ||
| ne : not examined | |||||||||
| N/B : no specific band | |||||||||
| N/D : specific band (50-65 kD) but no difference between WT and KO | |||||||||
| N/S : non-specific bands only | |||||||||
See here for vasopressin 1b receptor westerns.
Data as of 3/24/09
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