Section on Neural Gene Expression

Vasopressin 1b receptor antibodies

Various western studies performed in SNGE

(Heon-Jin Lee, Geetha Kannan, Matthew Morris, & Scott Young, unpublished)

			Source	Lolait lab	Santa Cruz Biotech	Life Span Biosciences	Apha Diagnostics	Santa Cruz Biotech	SDI
			Cat. #	n.e.	H-90 (SC-30026)	LS-A266	AVP1B13-A	D-20 (SC-18105)	Custom made
			Antigen origin	Rat	Human	Human	Rat	Mouse	Mouse
			Epitope location	C-terminus	N-terminus	N-terminus	N-terminus	N-terminus	C-terminus
			Antigen Seq.	395-412	181-270	n.e.	18 aa	n.e.	339-421
			Host	Rabbit	Rabbit	Rabbit	Rabbit	Goat	Rabbit
Western blot	BOILED METHODS	perfused	Liver	N/B	N/D	N/B	N/S	N/B	n.e.
		perfused	Pituitary	N/D	N/D	N/S	N/S	N/B	n.e.
		non-perfused	Liver	N/B	N/D	N/B	N/S	N/B	N/S
		non-perfused	Pituitary	N/D	N/D	N/D	N/S	N/B	N/S
	NON BOILED METHODS	perfused	Liver	n.e.	n.e.	n.e.	n.e.	n.e.	n.e.
		perfused	Pituitary	n.e.	n.e.	n.e.	n.e.	n.e.	n.e.
		non-perfused	Liver	N/B	n.e.	n.e.	n.e.	n.e.	N/B
			Hypothalamus	N/D	n.e.	n.e.	n.e.	n.e.	N/D
			Olfactory Bulb	N/D	n.e.	n.e.	n.e.	n.e.	N/B
			Pituitary	N/D	n.e.	n.e.	n.e.	n.e.	N/D
			Rat Pituitary	Specific	n.e.	n.e.	n.e.	n.e.	Specific
		Transfected CHO cells (Perkin-Elmer)		n.e.	n.e.	n.e.	n.e.	n.e.	Specific
		Epigen expression		n.e.	n.e.	n.e.	n.e.	n.e.	Specific

			ne : not examined
			N/B : no specific band
			N/D : specific band (50-65 kD) but no difference between WT and KO
			N/S : non-specific bands only

See here for oxytocin receptor westerns.
Data as of 3/24/09

The Section on Neural Gene Expression is in the Laboratory of Cellular and Molecular Regulation, Division of Intramural Research Programs is within the National Institute of Mental Health (NIMH) is a part the National Institutes of Health (NIH), and is a component of the U.S. Department of Health and Human Services.