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Photomicrograph shows oxytocin and vasopressin neurons within the human supraoptic nucleus labeled with 35S-labeled and digoxigenin-labeled probes, respectively. The arrow indicates a neuron containing both transcripts. Neurons containing the digoxigenin-labeled probe show a dark stain from development of the alkaline phosphatase on the anti-digoxigenin antibodies. The deposition of the radiolabeled probe is indicated by the green-colored silver grains. Note that a nucleus (n) is clearly delineated by the alkaline phosphatase staining. From unpublished work by Bruce Huang and W. Scott Young. |