Section on Neural Gene Expression
Table 2. Fluorochrome, horseradish peroxidase (HRP) and biotin conjugated secondary antibodies, biotinyl-tyramide, fluorochrome-tyramide and fluorochrome- or HRP-streptavidin conjugates.
| Type (source#)* | Conjugate (catalog#) | Dilution | Diluent | Fluorescent filter |
| Fluorochrome-conjugated secondary antibodies (1) | FITC-anti-rabbit IgG (711-096-152) | 1:100 | BSA diluent** | Green |
| Cy3-anti-rabbit IgG (711-166-152) | 1:1000 | BSA diluent** | Red | |
| FITC-anti-mouse IgG (715-096-151) | 1:100 | BSA diluent** | Green | |
| Cy3-anti-mouse IgG (715-166-151) | 1:1000 | BSA diluent** | Red | |
| HRP-conjugated secondary antibodies (1) | HRP-anti-rabbit IgG (711-096-152) | 1:1000 | BSA diluent** | - |
| HRP-anti-mouse IgG (715-166-151) | 1:1000 | BSA diluent** | - | |
| Biotin-conjugated secondary antibodies (2) | Biotin-anti-rabbit IgG (PK-6101) | 1:500 | BSA diluent** | - |
| Biotin-anti-mouse IgG (PK-6102) | 1:500 | BSA diluent** | - | |
| Biotinyl-tyramide (3) | Biotinyl-tyramide | 1:100 | Amplification diluent*** | - |
| Fluorochrome-tyramide conjugates (3) | FITC-tyramide | 1:2000 | Amplification diluent*** | Green |
| Rhodamine-tyramide | 1:5000 | Amplification diluent*** | Red | |
| Coumarin-tyramide | 1:2000 | Amplification diluent*** | UV | |
| Fluorochrome-streptavidin conjugates (3) | Streptavidin-FITC | 1:1000 | BSA diluent** | Green |
| Streptavidin-texas red | 1:2000 | BSA diluent** | Red | |
| Streptavidin-coumarin | 1:1000 | BSA diluent** | UV | |
| Streptavidin-HRP (3) | Streptavidin-HRP | 1:2000 | BSA diluent** | - |
*Sources: (1) Jackson ImmunoResearch, West Grove, PA; (2) Vector Laboratories, Burlingame, CA;
(3) DuPont NEN amplification kit, Wilmington, DE, catalog# NEL700
**BSA diluent: 1% bovine serum albumin, 0.6 v/v% Triton X-100 in 1x PBS, pH 7.4
***Amplification diluent: Diluted 1:1 with distilled water, from the 2x stock in the DuPont NEN kit
Immunohistochemistry pages.
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