Immunohistochemical Reagents

Table 2. Fluorochrome, horseradish peroxidase (HRP) and biotin conjugated secondary antibodies, biotinyl-tyramide, fluorochrome-tyramide and fluorochrome- or HRP-streptavidin conjugates.

Type (source#)^*	Conjugate (catalog#)	Dilution	Diluent	Fluorescent filter

Fluorochrome-conjugated secondary antibodies (1)	FITC-anti-rabbit IgG (711-096-152)	1:100	BSA diluent^**	Green
	Cy3-anti-rabbit IgG (711-166-152)	1:1000	BSA diluent^**	Red
	FITC-anti-mouse IgG (715-096-151)	1:100	BSA diluent^**	Green
	Cy3-anti-mouse IgG (715-166-151)	1:1000	BSA diluent^**	Red

HRP-conjugated secondary antibodies (1)	HRP-anti-rabbit IgG (711-096-152)	1:1000	BSA diluent^**	-
HRP-conjugated secondary antibodies (1)	HRP-anti-mouse IgG (715-166-151)	1:1000	BSA diluent^**	-

Biotin-conjugated secondary antibodies (2)	Biotin-anti-rabbit IgG (PK-6101)	1:500	BSA diluent^**	-
Biotin-conjugated secondary antibodies (2)	Biotin-anti-mouse IgG (PK-6102)	1:500	BSA diluent^**	-

Biotinyl-tyramide (3)	Biotinyl-tyramide	1:100	Amplification diluent^***	-

Fluorochrome-tyramide conjugates (3)	FITC-tyramide	1:2000	Amplification diluent^***	Green
	Rhodamine-tyramide	1:5000	Amplification diluent^***	Red
	Coumarin-tyramide	1:2000	Amplification diluent^***	UV

Fluorochrome-streptavidin conjugates (3)	Streptavidin-FITC	1:1000	BSA diluent^**	Green
	Streptavidin-texas red	1:2000	BSA diluent^**	Red
	Streptavidin-coumarin	1:1000	BSA diluent^**	UV

Streptavidin-HRP (3)	Streptavidin-HRP	1:2000	BSA diluent^**	-

^*Sources: (1) Jackson ImmunoResearch, West Grove, PA; (2) Vector Laboratories, Burlingame, CA; (3) DuPont NEN amplification kit, Wilmington, DE, catalog# NEL700
^**BSA diluent: 1% bovine serum albumin, 0.6 v/v% Triton X-100 in 1x PBS, pH 7.4
^***Amplification diluent: Diluted 1:1 with distilled water, from the 2x stock in the DuPont NEN kit

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