Section on Neural Gene Expression

Table 1. Schematic protocols of conventional and amplified immunostaining paths identified by letters in italic.

Path	Procedure		anti-IgG conjugate	Tertiary HRP^a conjugate	Tyramine (T) conjugate or DAB^c	Streptavidin (S) conjugate	Tyramine (T) conjugate or DAB^c

1	Con ven tion al	fluorescent	FITC, Cy3
2		peroxidase	HRP^a		DAB^c
3		peroxidase	Biotin	ABC^b	DAB^c

4	A m p l i f i e d	fluorescent	HRP^a		T-fluorochrome^d
5		fluorescent	HRP^a		T-biotin	S-fluorochrome^e
6		fluorescent	HRP^a		T-biotin	S-HRP^a	T-fluorochrome^d
7		peroxidase	HRP^a		T-biotin	S-HRP^a	DAB^c
8		fluorescent	Biotin	ABC^b	T-fluorochrome^d
9		fluorescent	Biotin	ABC^b	T-biotin	S-fluorochrome^e
10		fluorescent	Biotin	ABC^b	T-biotin	S-HRP^a	T-fluorochrome^d
11		peroxidase	Biotin	ABC^b	T-biotin	S-HRP^a	DAB^c
12		fluorescent, simple	FITC	alpha-FITC-HRP^a	T-fluorochrome^d
13		fluorescent, alternative	FITC	alpha-FITC-HRP^a	T-biotin	S-fluorochrome^e
14		fluorescent, forced	FITC	alpha-FITC-HRP^a	T-biotin	S-HRP^a	T-fluorochrome^d
15		peroxidase	FITC	alpha-FITC-HRP^a	T-biotin	S-HRP^a	DAB^c

^{a: Horseradish peroxidase

^bABC: Avidin-biotin-peroxidase complex from Vector Elite kit can be replaced by streptavidin-HRP

^cDAB: Diaminobenzidine

^dFluorochromes conjugated to tyramine: fluorescein, rhodamine, coumarin

^eFluorochromes conjugated to streptavidin: fluorescein, texas-red, coumarin}

Immunohistochemistry pages.

The Section on Neural Gene Expression is in the Laboratory of Cellular and Molecular Regulation, Division of Intramural Research Programs is within the National Institute of Mental Health (NIMH) is a part the National Institutes of Health (NIH), and is a component of the U.S. Department of Health and Human Services.