Section on Neural Gene Expression

Here are some of the protocols that we in the SNGE have adapted, developed, and/or used with success:

Immunohistochemistry
Comprehensive and annotated hybridization histochemistry
Y chromosome detection by hybridization histochemistry
Oligodeoxynucleotide labeling (3' or 5' ends)
RNA (riboprobe) labeling
Northern analysis (with oligonucleotide or riboprobes)
Autoradiography using Emulsion-coated Coverslips
Extraction and PCR Analyis of DNA from Mice Tails
Simple Miniprep DNA Preparation
Rapid DNA Prep for Restriction Analysis
Subcloning Protocol
Bacterial Artificial Chromosome (BAC) Protocols:

BAC Sequencing

BAC Preparation (gentle, best quality)

BAC Preparation (column, high quality)

RestrictionEnzyme^© analysis and prediction program. It was written in Pascal in our lab but has not been updated for the OS X. The source code is available so if you upgrade, please let me know!

The Section on Neural Gene Expression is in the Laboratory of Cellular and Molecular Regulation, Division of Intramural Research Programs is within the National Institute of Mental Health (NIMH) is a part the National Institutes of Health (NIH), and is a component of the U.S. Department of Health and Human Services.