Here are some of the protocols that we in the SNGE have adapted, developed, and/or used with success:
Immunohistochemistry
Comprehensive and annotated hybridization histochemistry
Y chromosome detection by hybridization histochemistry
Oligodeoxynucleotide labeling (3' or 5' ends)
RNA (riboprobe) labeling
Northern analysis (with oligonucleotide or riboprobes)
Autoradiography using Emulsion-coated Coverslips
Extraction and PCR Analyis of DNA from Mice Tails
Simple Miniprep DNA Preparation
Rapid DNA Prep for Restriction Analysis
Subcloning Protocol
Bacterial Artificial Chromosome (BAC) Protocols:
BAC Sequencing
BAC Preparation (gentle, best quality)
BAC Preparation (column, high quality)
RestrictionEnzyme
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Analysis and Prediction Program
Comments and suggestions are welcome!
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