Laboratory of Cell Biology1, National Institute of Mental Health and ÝLaboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health; and §Department of Experimental Zoology, Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel
J. Neuroendocrinol. 6: 917-925 1990
| A.Immunocytochemical staining of hypothalamic sections through
the paraventricular nucleus (PVN) of control (upper pair) and
transgenic (lower pair) mice. The rat transgene construct shown in B
was used to generate this transgenic mouse. Only cells in the
transgenic mouse PVN could be stained by the rat-specific
oxytocin-associated neurophysin antibody (PS 67). The other
oxytocin-associated neurophysin antibody (PS 38) which reacted
with both rat and mouse neurophysins, stained both PVN sections. B. Rat transgene construct. This construct contained both the intact VP and intact rat OT genes fused in tandem in the same transcriptional orientation and contained 1.4 kb and 0.36kb of the 5'-upstream flanking region of the rat VP and OT genes, respectively | 
|
1Now the Laboratory of Cellular and Molecular Regulation